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Fungsi lain yang menarik adalah peran intron dalam evolusi. Keberadaan intron yang terpisah-pisah dalam gen memungkinkan terjadinya rekombinasi genetik yang lebih sering. Ini bisa mempercepat proses evolusi dengan memungkinkan penciptaan gen-gen baru atau kombinasi alel yang menguntungkan. Selain itu, intron juga bisa menjadi tempat 'penyimpanan' bagi elemen genetik lain, seperti pseudogen atau transposon, yang juga bisa memiliki peran dalam dinamika genom dari waktu ke waktu.